Nitrous Oxide gas can affect the patient and also cause chronic complications in operating room staff in the short term. In the current survey, we measured the Nitrous Oxide gas levels in the operating room of the educational hospitals of the Ahvaz Jundishapur Medical University.576 samples were taken from 32 operating rooms through 4 months. Sampling was done in 3 different working times (8:30-9:00, 10:30-11, 12:30-13:00) from 6 different sites in the operating room. Portable infrared spectrophotometer was used for measuring the concentration of nitrous oxide.